Detection P53, Rb1 and H-ras Loss of Heterozygosity LOH in Patients with Urinary Bladder carcinoma

In this study 22 samples of urine sediments were obtained from patients with Urinary Bladder Carcinoma, DNA base technique and LOH detection was investigated in three genes P53, Rb1 and H-ras. The result are shown that LOH was present in most cases and for all genes where LOH in P53 represents (31.8%) and for Rb1 (45.4%) and for H-ras gene (27.2%).In some cases, more than one gene locus mutations were found

Bacteriological and immunological study associated with patients of Urinary Bladder carcinoma

The blood, serum & urine samples are collected from (22) patients with urinary bladder cancer were admitted to Al-Hilla general teaching hospital after diagnosis by helping urology physician. The result appeared that E.coli with high percentage (22.7% ,27.2%)in nixed &single infection respectively followed by Ps.aeuroginosa (22.7% , 13.6%) and S.aureus (18.8% , 9.09%).   Interleukin 2 &Interleukin 8 concentration level was detected in serum by enzyme-linked immunosorbent assay(ELISA) and data were compared with control to compare the performance of each cytokins & results show no elevated , no significant decrease in level  in two cytokins, this leads  to an independent factors for the detection of urinary bladder cancer

Molecular Study on Distrbution of Sul-1 and Sul-2 genes among Salmonella enterica causing Enteric Fever

This study comprised 200 blood samples taken from patients visited some of Al-Najaf province. The most prevalent species was Salmonella Typhi, where 20  isolates had been identified. Serological typing was done by using both poly and monovalent antisera, which revealed that all isolated Salmonella was belong to serovar Typhi. DNA was extracted from all Salmonella Typhi isolates. Extracted DNA was used in Polymerase Chain Reaction technique. Three pairs of primers was used to detect the presence of targeted genes (two for sul-1 gene, and one for sul-2) . Data showed the occurrence of sul-1 gene the first sequence in 15% of total , while second sequence gave no positive result. Sul-2 gene on the other hand , showed 50% positivity.

Integration of pharmacist independent prescribers into general practice: a mixed-methods study of pharmacists’ and patients’ views

Abstract Background Since 2015, the National Health Service (NHS) has funded pharmacists to work in general practice (GP practice) to ease workload pressures. This requires pharmacists to work in new roles and be integrated effectively in GPs. Independent prescribing is a key part of the GP pharmacist role, but little is known about pharmacists’ integration into GP practice as well as patients’ perceptions and experiences of the care provided by GP pharmacists. This study aims to explore the perceptions of pharmacist independent prescribers (PIPs) about their integration into GP practice and gain insight into patients’ perceptions about the care provided to them by pharmacists. Methods A mixed-methods study comprising semi-structured interviews with PIPs (n = 13) followed by questionnaire-based assessment of patients’ (n = 77) evaluation of pharmacists’ care was conducted between December 2019 and March 2020. Quantitative data were analysed using descriptive statistics. Interviews and open comments of the survey were thematically analysed. Results Pharmacist independent prescribers reported undertaking a range of patient-facing and non-clinical roles. Lack of understanding about PIPs’ clinical role and working beyond their clinical area of competence were some of the barriers to their integration into GP practice. Most patients were satisfied with the consultations they received from pharmacists and reported confidence in the pharmacist’s recommendations about their health conditions. However, a few patients (14%) felt they would still need to consult a general practitioner after their appointment and 11% were not sure if a further consultation was needed. Conclusions Pharmacist independent prescribers provide a range of clinical services for the management of long-term conditions which appear to be recognised by patients. However, there is a need to address the barriers to PIPs’ integration into GP practice to optimise their skill-mix and patient-centred care

Human Salmonellosis: A Continuous Global Threat in the Farm-to-Fork Food Safety Continuum

Salmonella is one of the most common zoonotic foodborne pathogens and a worldwide public health threat. Salmonella enterica is the most pathogenic among Salmonella species, comprising over 2500 serovars. It causes typhoid fever and gastroenteritis, and the serovars responsible for the later disease are known as non-typhoidal Salmonella (NTS). Salmonella transmission to humans happens along the farm-to-fork continuum via contaminated animal- and plant-derived foods, including poultry, eggs, fish, pork, beef, vegetables, fruits, nuts, and flour. Several virulence factors have been recognized to play a vital role in attaching, invading, and evading the host defense system. These factors include capsule, adhesion proteins, flagella, plasmids, and type III secretion systems that are encoded on the Salmonella pathogenicity islands. The increased global prevalence of NTS serovars in recent years indicates that the control approaches centered on alleviating the food animals’ contamination along the food chain have been unsuccessful. Moreover, the emergence of antibiotic-resistant Salmonella variants suggests a potential food safety crisis. This review summarizes the current state of the knowledge on the nomenclature, microbiological features, virulence factors, and the mechanism of antimicrobial resistance of Salmonella. Furthermore, it provides insights into the pathogenesis and epidemiology of Salmonella infections. The recent outbreaks of salmonellosis reported in different clinical settings and geographical regions, including Africa, the Middle East and North Africa, Latin America, Europe, and the USA in the farm-to-fork continuum, are also highlighted